primary rat pulmonary artery endothelial cells Search Results


primary rat pulmonary artery endothelial cells  (Dawley Inc)
90
Dawley Inc primary rat pulmonary artery endothelial cells
Primary Rat Pulmonary Artery Endothelial Cells, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary rat pulmonary artery endothelial cells/product/Dawley Inc
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primary rat pulmonary artery endothelial cells - by Bioz Stars, 2026-03
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rat pulmonary arterial endothelial cells  (Dawley Inc)
90
Dawley Inc rat pulmonary arterial endothelial cells
METTL3 was gradually upregulated in hypoxia-induced <t>rPAECs.</t> (a) The primary rPAECs isolated from male Sprague-Dawley rats were identified using immunofluorescence. rPAECs were exposed to hypoxic conditions (Hx, 1% O 2 ) for 6 h, 12 h, 24 h, and 48 h. (b) The migration of rPAECs was determined by the transwell assay. (c) Western blot examined the protein levels of CD31, VE-cadherin, α -SMA, and vimentin. (d, e) The mRNA and protein levels of METTL3 were determined by RT-qPCR and western blot. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.
Rat Pulmonary Arterial Endothelial Cells, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat pulmonary arterial endothelial cells/product/Dawley Inc
Average 90 stars, based on 1 article reviews
rat pulmonary arterial endothelial cells - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
rat pulmonary artery endothelial cells (rpaecs)  (Creative Bioarray Inc)
90
Creative Bioarray Inc rat pulmonary artery endothelial cells (rpaecs)
METTL3 was gradually upregulated in hypoxia-induced <t>rPAECs.</t> (a) The primary rPAECs isolated from male Sprague-Dawley rats were identified using immunofluorescence. rPAECs were exposed to hypoxic conditions (Hx, 1% O 2 ) for 6 h, 12 h, 24 h, and 48 h. (b) The migration of rPAECs was determined by the transwell assay. (c) Western blot examined the protein levels of CD31, VE-cadherin, α -SMA, and vimentin. (d, e) The mRNA and protein levels of METTL3 were determined by RT-qPCR and western blot. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.
Rat Pulmonary Artery Endothelial Cells (Rpaecs), supplied by Creative Bioarray Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat pulmonary artery endothelial cells (rpaecs)/product/Creative Bioarray Inc
Average 90 stars, based on 1 article reviews
rat pulmonary artery endothelial cells (rpaecs) - by Bioz Stars, 2026-03
90/100 stars
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primary rat pulmonary artery endothelial cell culture  (Simonsen Laboratories)
90
Simonsen Laboratories primary rat pulmonary artery endothelial cell culture
METTL3 was gradually upregulated in hypoxia-induced <t>rPAECs.</t> (a) The primary rPAECs isolated from male Sprague-Dawley rats were identified using immunofluorescence. rPAECs were exposed to hypoxic conditions (Hx, 1% O 2 ) for 6 h, 12 h, 24 h, and 48 h. (b) The migration of rPAECs was determined by the transwell assay. (c) Western blot examined the protein levels of CD31, VE-cadherin, α -SMA, and vimentin. (d, e) The mRNA and protein levels of METTL3 were determined by RT-qPCR and western blot. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.
Primary Rat Pulmonary Artery Endothelial Cell Culture, supplied by Simonsen Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary rat pulmonary artery endothelial cell culture/product/Simonsen Laboratories
Average 90 stars, based on 1 article reviews
primary rat pulmonary artery endothelial cell culture - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


METTL3 was gradually upregulated in hypoxia-induced rPAECs. (a) The primary rPAECs isolated from male Sprague-Dawley rats were identified using immunofluorescence. rPAECs were exposed to hypoxic conditions (Hx, 1% O 2 ) for 6 h, 12 h, 24 h, and 48 h. (b) The migration of rPAECs was determined by the transwell assay. (c) Western blot examined the protein levels of CD31, VE-cadherin, α -SMA, and vimentin. (d, e) The mRNA and protein levels of METTL3 were determined by RT-qPCR and western blot. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: METTL3 Promotes Endothelium-Mesenchymal Transition of Pulmonary Artery Endothelial Cells by Regulating TRPC6/Calcineurin/NFAT Signaling Pathways

doi: 10.1155/2023/8269356

Figure Lengend Snippet: METTL3 was gradually upregulated in hypoxia-induced rPAECs. (a) The primary rPAECs isolated from male Sprague-Dawley rats were identified using immunofluorescence. rPAECs were exposed to hypoxic conditions (Hx, 1% O 2 ) for 6 h, 12 h, 24 h, and 48 h. (b) The migration of rPAECs was determined by the transwell assay. (c) Western blot examined the protein levels of CD31, VE-cadherin, α -SMA, and vimentin. (d, e) The mRNA and protein levels of METTL3 were determined by RT-qPCR and western blot. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.

Article Snippet: Primary rat pulmonary arterial endothelial cells (rPAECs) were isolated from Sprague-Dawley rats and verified by CD31 immunofluorescence staining. rPAECs were exposed to hypoxic conditions to induce EndMT.

Techniques: Isolation, Immunofluorescence, Migration, Transwell Assay, Western Blot, Quantitative RT-PCR

Downregulation of METTL3 suppressed the hypoxia-induced EndMT process. (a, b) Lentivirus-mediated shMETTL3 and shNC were transfected into rPAECs; then, the mRNA and protein levels of METTL3 were determined by RT-qPCR and western blot. lv-shMETTL3- and lv-shNC-transfected cells were exposed to hypoxic conditions. (c) Western blot detected the METTL3 protein level. (d) The migration of rPAECs was evaluated by the transwell assay. (e) Western blot examined the levels of CD31, VE-cadherin, α -SMA, and vimentin. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: METTL3 Promotes Endothelium-Mesenchymal Transition of Pulmonary Artery Endothelial Cells by Regulating TRPC6/Calcineurin/NFAT Signaling Pathways

doi: 10.1155/2023/8269356

Figure Lengend Snippet: Downregulation of METTL3 suppressed the hypoxia-induced EndMT process. (a, b) Lentivirus-mediated shMETTL3 and shNC were transfected into rPAECs; then, the mRNA and protein levels of METTL3 were determined by RT-qPCR and western blot. lv-shMETTL3- and lv-shNC-transfected cells were exposed to hypoxic conditions. (c) Western blot detected the METTL3 protein level. (d) The migration of rPAECs was evaluated by the transwell assay. (e) Western blot examined the levels of CD31, VE-cadherin, α -SMA, and vimentin. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.

Article Snippet: Primary rat pulmonary arterial endothelial cells (rPAECs) were isolated from Sprague-Dawley rats and verified by CD31 immunofluorescence staining. rPAECs were exposed to hypoxic conditions to induce EndMT.

Techniques: Transfection, Quantitative RT-PCR, Western Blot, Migration, Transwell Assay

METTL3 activated calcineurin/NFAT signaling via promoting TRPC6. rPAECs were divided into the following groups: Nx, Hx, Hx + lv-shNC, Hx + lv-shMETTL3, Hx + HF, and Hx + lv-shMETTL3 + HF. After indicated treatment, the subsequent experiments were conducted. (a) Western blot assessed the protein level of TRPC6. (b) The changes in calcineurin activity were detected by using the commercial kit. (c) The transcriptional activity of NFAT was detected by the luciferase assay. (d) Western blot quantified the levels of NFATc1, NFATc2, NFATc3, and NFATc4. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: METTL3 Promotes Endothelium-Mesenchymal Transition of Pulmonary Artery Endothelial Cells by Regulating TRPC6/Calcineurin/NFAT Signaling Pathways

doi: 10.1155/2023/8269356

Figure Lengend Snippet: METTL3 activated calcineurin/NFAT signaling via promoting TRPC6. rPAECs were divided into the following groups: Nx, Hx, Hx + lv-shNC, Hx + lv-shMETTL3, Hx + HF, and Hx + lv-shMETTL3 + HF. After indicated treatment, the subsequent experiments were conducted. (a) Western blot assessed the protein level of TRPC6. (b) The changes in calcineurin activity were detected by using the commercial kit. (c) The transcriptional activity of NFAT was detected by the luciferase assay. (d) Western blot quantified the levels of NFATc1, NFATc2, NFATc3, and NFATc4. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.

Article Snippet: Primary rat pulmonary arterial endothelial cells (rPAECs) were isolated from Sprague-Dawley rats and verified by CD31 immunofluorescence staining. rPAECs were exposed to hypoxic conditions to induce EndMT.

Techniques: Western Blot, Activity Assay, Luciferase

Activation of TRPC6/calcineurin/NFAT pathways on the biological roles of METTL3 knockdown in hypoxia-induced rPAECs. rPAECs were divided into the following groups: Nx, Hx, Hx + lv-shMETTL3, Hx + lv-shMETTL3+HF, and Hx + lv-shMETTL3 + ET-1. After indicated treatment, the subsequent experiments were conducted. (a) The migration of rPAECs was tested by the transwell assay. (b) Western blot examined the levels of CD31, VE-cadherin, α -SMA, and vimentin. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: METTL3 Promotes Endothelium-Mesenchymal Transition of Pulmonary Artery Endothelial Cells by Regulating TRPC6/Calcineurin/NFAT Signaling Pathways

doi: 10.1155/2023/8269356

Figure Lengend Snippet: Activation of TRPC6/calcineurin/NFAT pathways on the biological roles of METTL3 knockdown in hypoxia-induced rPAECs. rPAECs were divided into the following groups: Nx, Hx, Hx + lv-shMETTL3, Hx + lv-shMETTL3+HF, and Hx + lv-shMETTL3 + ET-1. After indicated treatment, the subsequent experiments were conducted. (a) The migration of rPAECs was tested by the transwell assay. (b) Western blot examined the levels of CD31, VE-cadherin, α -SMA, and vimentin. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.

Article Snippet: Primary rat pulmonary arterial endothelial cells (rPAECs) were isolated from Sprague-Dawley rats and verified by CD31 immunofluorescence staining. rPAECs were exposed to hypoxic conditions to induce EndMT.

Techniques: Activation Assay, Knockdown, Migration, Transwell Assay, Western Blot